PCR試劑 流感病毒AB/合胞病毒AB四聯(lián)檢測(cè)試劑盒

廣州健侖生物科技有限公司

One tube multiplex for detection of influenza A, B, respiratory syncytial virus A/B and internal control.
一管多重檢測(cè)流感A，B，呼吸道合胞病毒A / B和內(nèi)部控制。
Principle
Multiplex real-time PCR for detection of pathogen genes by TaqMan® technology
Targets
One tube multiplex PCR:
influenza A virus
influenza B virus
human respiratory syncytial viruses A/B
internal Control
Specimen
This test is for use with extracted nucleic acid from respiratory samples (throat/nasal swabs, bronchoalveolar lavage and sputum) of human origin
Storage
-20°C
Shelf life
12 months from manufacture

PCR試劑 流感病毒AB/合胞病毒AB四聯(lián)檢測(cè)試劑盒

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PCR試劑

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

9．封閉：在山羊血清封閉，非特異性染色仍然較強(qiáng)時(shí)，可延長(zhǎng)封閉時(shí)間或用濃縮血清封閉 
10．抗體稀釋：應(yīng)遵循“現(xiàn)用現(xiàn)配”的原則，對(duì)于PBS稀釋的抗體一定要當(dāng)天使用。 
11．背景高：在抗體濃度、反應(yīng)時(shí)間、反應(yīng)溫度等合適的條件下，如果背景依舊高，可采用含1‰ Tween20的PBS洗，特別是在顯色之前要多洗。 
12．返藍(lán)：在蘇木素復(fù)染后，可用堿性緩沖液（如PBS）或Na2HPO4的飽和溶液返藍(lán)。 
13．顯色：一定要在顯微鏡下觀察，注意控制背景。
DAB法 顯示過(guò)氧化物酶
〔原理〕  過(guò)氧化物酶分解H2O2的過(guò)程中，下面反應(yīng)不直接發(fā)生：AH2（供氫體）+H2O2→A（供氫體的氧化物）+2H2O2實(shí)驗(yàn)可知，有稱為復(fù)合物 Ⅰ、Ⅱ、Ⅲ、的酶——底物（受氫體）復(fù)合體生成，在復(fù)合體zui后分解為酶和水時(shí)，游離的原子氧使供氫體氧化而顯色。酶組織化學(xué)中所使用的供氫體應(yīng)是含有色原性基團(tuán)的發(fā)色物質(zhì)，如奈酚和聯(lián)苯胺。
免疫組化染色步驟（以ABC法為例）

9. Blocking: When goat serum is closed and nonspecific staining is still strong, it can be extended or closed with concentrated serum
10. Antibody dilution: Follow the principle of "Ready-to-use Ready-to-use". Antibody diluted with PBS must be used on the same day.
11. Background High: If the background is still high, under appropriate conditions of antibody concentration, reaction time, reaction temperature, etc., wash with PBS containing 1 ‰ Tween20, especially before developing.
12. Back to blue: In the hematoxylin counterstain, available alkaline buffer (such as PBS) or Na2HPO4 saturated solution back to blue.
13. Color: Be sure to observe under the microscope, pay attention to control the background.
DAB method shows peroxidase
[Principle] In the process of decomposition of H2O2 by peroxidase, the following reaction does not directly occur: AH2 (hydrogen donor) + H2O2 → A (hydrogen donor oxide) + 2H2O2 experiment shows that there are complex Ⅰ, Ⅱ , Ⅲ, the enzyme (substrate) (substrate) is generated by the complex, the final decomposition of the complex enzyme and water, the free atomic oxygen oxidation of hydrogen donor and color. Hydrogen donors used in enzymatic histochemistry should be chromogenic substances containing chromogenic groups, such as naloxol and benzidine.
Immunohistochemical staining steps (ABC method as an example)