EIKEN食物感染流感病毒快速檢測(cè)試劑盒

廣州健侖生物科技有限公司

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EIKEN食物感染流感病毒快速檢測(cè)試劑盒

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

一、無(wú)色片
染色結(jié)束后，切片中見(jiàn)不到任何陽(yáng)性信號(hào)。這是常規(guī)工作中比較常見(jiàn)的現(xiàn)象，出現(xiàn)這種現(xiàn)象，有兩種可能：1、真陰性結(jié)果：整個(gè)染色過(guò)程沒(méi)有出現(xiàn)問(wèn)題，組織或細(xì)胞確實(shí)不表達(dá)與抗體相關(guān)的抗原。2、假陰性結(jié)果：即此陰性結(jié)果不是真實(shí)的反映。假陰性結(jié)果又可分為兩種情況：（1）切片中根本就不包含所預(yù)期檢查的組織或細(xì)胞。出現(xiàn)這種情況，要麼是病理醫(yī)生選擇錯(cuò)了切片或抗體選錯(cuò)了，要麼是技術(shù)員選錯(cuò)了蠟塊。獲得正確的切片進(jìn)行染色是獲得正確結(jié)果的前提。由此表明：制作出合格的免疫組化切片不僅僅是技術(shù)員的事，病理醫(yī)生也起著*的作用。（2）染色過(guò)程中的某一或某些環(huán)節(jié)出了問(wèn)題。比如，組織未進(jìn)行抗原修復(fù)，有的組織必須經(jīng)過(guò)抗原修復(fù)才能檢測(cè)抗原表達(dá)；或選用了只能用于冰凍組織而不能用于石蠟包埋組織的抗體；或一抗失效，雖然抗體失效在理論上是一個(gè)逐漸的過(guò)，但偶爾也遇到突然失效的情況，抗體長(zhǎng)期不用和/或已超過(guò)有效期是主要的原因。也可見(jiàn)于染色過(guò)程中漏掉了某一環(huán)節(jié)，如忘記加二抗或三抗，或用了兩次二抗而缺少了三抗，或配制DAB時(shí)少了*。為了避免這種簡(jiǎn)單的錯(cuò)誤，有一種簡(jiǎn)單的方法：在三抗孵育結(jié)束時(shí)，將切片上的三抗甩在一張白紙上，在將配制好的DAB 滴一滴在白紙的三抗上，觀察是否出現(xiàn)棕色。如果出現(xiàn)了，證明三抗和DAB的配制過(guò)程沒(méi)有錯(cuò)誤。如果這種DAB再滴到切片上沒(méi)有出現(xiàn)任何陽(yáng)性信號(hào)，問(wèn)題一定是出在三抗以前。如果紙上不出現(xiàn)棕色反應(yīng)，問(wèn)題肯定在三抗或DAB的配制過(guò)程。這種簡(jiǎn)單方法能迅速的幫助我們查找出現(xiàn)問(wèn)題可能的原因。

First, colorless film
After staining, no positive signals were seen in the sections. This is a common phenomenon in routine work. There are two possibilities for this phenomenon: 1. True negative result: There is no problem in the whole dyeing process, and the tissue or cells do not express the antibody related to the antibody. 2, false negative results: This negative result is not a true reflection. False negative results can be divided into two cases: (1) The slice does not contain the tissue or cells to be examined at all. When this happens either the pathologist chooses the wrong slice or the antibody is the wrong one, or the technician selects the wrong wax block. Getting the right slice for staining is a prerequisite for getting the right result. This shows that: to produce qualified immunohistochemical sections is not just a technician, pathologists also play an indispensable role. (2) dyeing process in some or some of the problems. For example, the tissue is not antigenic repair, and some organizations must undergo antigen retrieval in order to detect antigen expression; or can only be used for frozen tissue can not be used for paraffin-embedded tissue antibodies; or primary antibody failure, although the antibody failure in the theory It is a gradual, but occasionally encountered a sudden failure, the antibody long-term useless and / or has expired is the main reason. Can also be found in the dyeing process missed a link, such as forgetting to add two or three anti-antibody, or the use of two secondary antibodies and the lack of the three anti-DAB preparation or less hydrogen peroxide. In order to avoid such a simple error, there is a simple way: in the third anti-incubation at the end of the triad anti-rejection on a piece of white paper, the prepared DAB drops of white on the three anti-drop , Observe whether there is brown. If present, it is demonstrated that there is no error in the formulation of the third antibody and DAB. If this DAB drop to the section did not appear any positive signal, the problem must be out before the third antibody. If the paper does not appear brown reaction, the problem is certainly in the third antibody or DAB preparation process. This simple method quickly helps us find out the probable cause of the problem.